Comparison of the rapid automated MTT-assay with a dye exclusion assay for chemosensitivity testing in childhood leukaemia
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چکیده
منابع مشابه
Comparison of dye exclusion assays with a clonogenic assay in the determination of drug-induced cytotoxicity.
The following factors must be considered when dye exclusion assays are interpreted. (a) It may require several days for lethally damaged cells to lose their membrane integrity following a cytotoxic insult. (b) During this time, the "surviving" cells may continue to proliferate. (c) Also during this time, some lethally damaged cells may undergo an early disintegration, so that they are not prese...
متن کاملComparison of Dye Exclusion Assays with a Clonogenic Assay in the Determination of Drug-induced Cytotoxicity1
The following factors must be considered when dye exclu sion assays are interpreted, (a) It may require several days for lethally damaged cells to lose their membrane integrity follow ing a cytotoxic insult, (b) During this time, the "surviving" cells may continue to proliferate, (c) Also during this time, some lethally damaged cells may undergo an early disintegration, so that they are not pre...
متن کاملMTT colorimetric assay for testing macrophage cytotoxic activity in vitro.
The MTT (3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide) cleavage assay, originally described by Mosmann (1983, J. Immunol. Methods 65, 55) for measuring cell survival/proliferation, has been used successfully to quantitate macrophage-mediated cytotoxicity. Peritoneal macrophages, from control or pyran copolymer MVE2-treated mice and from TU5 and L929 murine cell lines, were used ...
متن کاملEvaluation of chemosensitivity testing with highly purified tumor cells in 435 patients with gastric carcinoma using an MTT assay.
BACKGROUND We evaluated the results of chemosensitivity testing for gastric cancer using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay in terms of the correlation of chemosensitivity and clinicopathological findings. PATIENTS AND METHODS We analyzed 435 consecutive patients with gastric cancer treated between January 1991 and January 2002. Highly purified fresh h...
متن کاملRapid fluorescence-based assay for radiosensitivity and chemosensitivity testing in mammalian cells in vitro.
An efficient and rapid cytotoxicity assay has been developed, particularly for radiobiological studies, utilizing 96-well microtiter plates. Several days after treatment, cell numbers per well were measured by fluorescent intensity using an automatic reader after staining with the DNA specific dye Hoechst 33258. For radiobiological applications, a microtiter plate irradiation box was designed a...
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ژورنال
عنوان ژورنال: British Journal of Cancer
سال: 1989
ISSN: 0007-0920,1532-1827
DOI: 10.1038/bjc.1989.44